A couple hundred pages ago in this thread I wanted to start a rumor that vaping lung disease was actually Sars-CoV2 laced in black market Chinese vape carts.
https://www.cdc.gov/coronavirus/2019...oratories.html
https://www.cdc.gov/coronavirus/2019...fety-faqs.html
Much of this is directly disrtibuted to Clinical labs thru the CDC. BSL2 is what is required, what we have at work for handling samples.
FYI- the rates of False Positives being tossed around in this thread are nonsense without the understanding the there is NOT a universal rt-PCR assay, but at least 10 different assays, all with their own primer sets, that have been developed. Early versions of the Chinese assay and the CDC assay both suffered from FP, which were corrected in later versions. The test developed early on in Germany and adopted by the WHO has a very low FP rate, as do the 4 different assays developed in Korea and used on nearly 300,000 people in the country thus far.
https://www.who.int/emergencies/dise...atory-guidance
3. Molecular assays to diagnose 2019-nCoV
Several assays that detect the 2019-nCoV have been and are currently under development, both in-house and commercially. Some assays may detect only the novel virus and some may also detect other strains (e.g. SARS-CoV) that are genetically similar.
In-house developed molecular assays
Some groups shared their protocols which can be accessed below (Summary table and link to the protocols). In some cases, the groups will be willing to send reagents or reagent mixes prepared in their laboratories, with or without associated fees. It is strongly recommended to contact the developer if you need further assistance. The list is not exhaustive and is being updated regularly.
Summary table of available protocols
Country Institute Gene targets China China CDC ORF1ab and N Germany Charité RdRP, E, N Hong Kong SAR HKU ORF1b-nsp14, N Japan National Institute of Infectious Diseases, Department of Virology III Pancorona and multiple targets, Spike protein Thailand National Institute of Health N US US CDC Three targets in N gene France Institut Pasteur, Paris Two targets in RdRP