No. There is no airflow over the lifting surface.
0 Knots True Airspeed.
It's Ground Speed could be 400KTA relative to the tread mill, but as long as there is 0KTA over the airfoil, it can't take off.
That's why planes take-off and land into the wind. While the plane may be fighting a head wind and have a lower ground speed, its airspeed is higher by however many knots the headwind is.
for example: at 200kts ground speed in a 10kt headwind, the kta is 210kts. and off into the wild blue yonder you go.
Morans.
http://en.wikipedia.org/wiki/Airfoil